Effects of Y-27632 or toxin B on CCL19-induced dendritic extension of mature DCs.

BC1 cells were activated by LPS and used as mature DCs. The mature DCs were pretreated with Y-27632 (10 μM), a Rho-associated kinase inhibitor, or toxin B (4 ng/mL), a Rho GTPase inhibitor, for 5 hours and then incubated with CCL19 (20 ng/mL) for 2 hours. The morphologic changes were analyzed by invert microscopy (original magnification × 20). The results are representative of 4 independent experiments (A). Each column represents the mean ± SE of 4 independent experiments (B).