Immunofluorescence analysis of γ-globin expression in RBCs.

Examples of immunofluorescence analysis of γ-globin expression in RBCs of mice receiving marrow transduced with vector HS40-11. (A) Two-step staining of typical blood smear with anti-γ followed by anti-mouse FITC showing variation in level of expression. Original magnification × 50. (B) Flow cytometry analysis for 5 independent recipients and one control animal containing a μLCR-γ transgene.43 The percentage of γ-positive RBCs in the experimental samples (heavy line) is reported above the indicated gates after subtracting the background from the mock-transduced control (filled histograms). x-axis, log relative fluorescence; y-axis, cell number.