American Society of Hematology

Fig. 4.

$Fig. 4. Normalized γ-globin expression in long-term reconstituted mice. / Southern blot analysis was performed on spleens collected at the time of death (typically 6-7 months after transplantation), and the copies of provirus per genome were determined. The fraction of hematopoietic cells containing at least one copy per cell was then calculated using the Poisson distribution (assuming the provirus was distributed randomly). This frequency was then used to normalize the fraction of RBCs expressing γ-globin at the latest time points presented in Figure 3. Each point on the scatter plot shows the fraction of RBCs expressing human γ-globin divided by the calculated fraction of cells containing provirus for individual mice.$

Normalized γ-globin expression in long-term reconstituted mice.

Southern blot analysis was performed on spleens collected at the time of death (typically 6-7 months after transplantation), and the copies of provirus per genome were determined. The fraction of hematopoietic cells containing at least one copy per cell was then calculated using the Poisson distribution (assuming the provirus was distributed randomly). This frequency was then used to normalize the fraction of RBCs expressing γ-globin at the latest time points presented in Figure 3. Each point on the scatter plot shows the fraction of RBCs expressing human γ-globin divided by the calculated fraction of cells containing provirus for individual mice.

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