Fig. 6.
Competitive repopulation assay.
Lethally irradiated B6 mice were injected with 7.5 × 106B6GFP BM cells competed against either 7.5 × 106nonirradiated B6Ly5.2 BM cells (n = 7) or 7.5 × 106B6Ly5.2 BM cells that had been irradiated with 276 cGy in vivo (n = 7). Donor chimerism resulting from the GFP and nonirradiated or irradiated B6Ly5.2 BM cells was assessed at 1, 2, and 4 months after transplantation (A). At 4 months after transplantation, lineage analysis of both donor cell populations (B6GFP and irradiated or nonirradiated B6Ly5.2) was performed (B).
