The depletion of CD4⁺ T cells found in pIV^−/− mice is comparable to that observed in Aα^−/− mice.

CD4⁺ and CD8⁺ T-cell populations were analyzed by FACS. Numbers indicate the percentage of CD4⁺, CD8⁺, and CD4⁺CD8⁺ cells. (A) Representative FACS analysis of thymocytes from pIV^+/−, pIV^−/− and I-Aα^−/− mice. (B) % of CD4⁺ and CD8⁺ T-cell populations from the lymph nodes, spleen, and PBLs were analyzed for pIV^−/− mice and pIV^+/− control littermates. (C) Representative FACS analysis showing the CD4⁺ and CD8⁺ T-cell populations in lymph nodes from control pIV^+/−, pIV^−/−, and I-Aα^−/−mice.