Quantitation of complementation of the

smf1/smf2 yeast mutant by Nramp2 variants. Individual Nramp2 mutants (identified) together with positive (Nramp2) and negative (pVT) controls were grown to saturation and diluted to OD₅₉₅ = 0.02 in either YPD or YPD pH 7.9. Cells were seeded in 96-well plates, and growth was measured after 24 hours' incubation at 30°C by measuring OD₅₉₅. Results represent relative growth (expressed as a percentage) of individual mutant grown at pH 7.9 versus normal YPD. Error bars represent standard deviations of the means.