The effect of CD18 blockade on I/R injury diminished with increasing ischemic times.

Mouse hind limb was subjected to varying periods of ischemia by aortic cross-clamping followed by 3 hours of reperfusion. CK concentration in plasma was determined following 3 hours of reperfusion following varying ischemic times. Solid bars show results for mice pretreated with CD18 mAb (2 μg/g body weight, administered intravenously, at 16 hours prior to cross-clamping) and open bars show results for controls treated with hamster IgG (2 μg/g body weight). Values represent means ± SEM. *P < .05 vs IgG-pretreated.