![Fig. 5. Allogeneic MLR-stimulating capacity of ES-DCs. / (A) Graded numbers of ES-DCs harvested on day 17 (⋄), ES-derived cells on day 10 (■), and (CBA × C57BL/6) F1 BM-DCs cultured for 11 days (○) were irradiated and cocultured with splenic T cells (1.5 × 105) isolated from Balb/c mice for 4 days, and the proliferative response of T cells in the last 16 hours of the culture was measured by [3H]-thymidine uptake. (B) ES-DCs harvested on day 24 and stimulated with IL-4/TNF-α/anti-CD40 (⋄), IL-4/TNF-α/LPS (▵), or IL-4/TNF-α/anti-CD40/LPS (▪) were used as stimulators. BM-DCs cultured for 10 days and stimulated with TNF-α (○) for 20 hours were also used as stimulators. For control, ES-derived cells cultured on OP9 feeder cell layers for 10 days were also used as stimulators (■). Mean values in kilo cpm (kcpm) ± standard deviation for triplicate cultures are shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/9/10.1182_blood-2002-07-2254/4/h80934224005.jpeg?Expires=1724262234&Signature=2~LNKzxNUmsiNl6UhMdp47rumbqHdDximNG3yUpE3z5Nl7JkSlSF37tmLH4l0cFQi185p2szkxjpx2xXpK7Q2SNSAcgSb8dDw8Z8~aD6Oercj2WY0EBBpN1zkxyXm0ZeQZqGuaZnIXaByefjDNiycKr5llIp2aO4GqFgtQYZCOJ3M-rjjp9eZLE2qN9MPrhS3Gm6lSWUbbBYd5y3x8LAVavic5MaQa5-2xRYdyiezcQPIlsQvM3Fk6G6wkZ2dZzZ0BIr2N-gd9M02QyLRjuCBx2Eto7edKLNJp987JI6NC8Jxms8WtBnjcRMdJLp6kFin0Rpmv1n01UT1jBZwlAQEg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Allogeneic MLR-stimulating capacity of ES-DCs.
(A) Graded numbers of ES-DCs harvested on day 17 (⋄), ES-derived cells on day 10 (■), and (CBA × C57BL/6) F1 BM-DCs cultured for 11 days (○) were irradiated and cocultured with splenic T cells (1.5 × 105) isolated from Balb/c mice for 4 days, and the proliferative response of T cells in the last 16 hours of the culture was measured by [3H]-thymidine uptake. (B) ES-DCs harvested on day 24 and stimulated with IL-4/TNF-α/anti-CD40 (⋄), IL-4/TNF-α/LPS (▵), or IL-4/TNF-α/anti-CD40/LPS (▪) were used as stimulators. BM-DCs cultured for 10 days and stimulated with TNF-α (○) for 20 hours were also used as stimulators. For control, ES-derived cells cultured on OP9 feeder cell layers for 10 days were also used as stimulators (■). Mean values in kilo cpm (kcpm) ± standard deviation for triplicate cultures are shown.
