Morphology of ES cell–derived DCs.

ES cell–derived cells on day 8 (A), day 12 (B-C), day 17 (D-E), and day 27 (F) of differentiation culture are shown. Cells on day 24 were recovered and stimulated for 2 days with IL-4, TNF-α, plus agonistic anti-CD40 mAb (G), or with IL-4, TNF-α, plus LPS (H). Panels A and B are phase-contrast micrographs. Scale bars represent 20 μm.