Hematopoietic differentiation of TT2 ES cells on feeder cell layers of OP9.

(A-B) Phase-contrast micrographs of TT2 ES cell colonies on OP9 feeder cell layers on day 3 (A) and day 5 (B) are shown. (C-E) May-Giemsa staining of cytospin specimens of hematopoietic cells derived from TT2 ES cells. TT2 cells were cultured on OP9 feeder cell layer for 15 days in total, without addition of exogenous cytokines. Floating cells were applied to cytospin preparations and stained with May-Giemsa. Cells of myeloid (C), erythroid (D), and megakaryocytic (E) lineages are shown. Scale bars represent 50 μm (A-B); and 20 μm, (C-E).