Allostimulatory capacity of sort purified Lin⁻ cell subpopulations.

Varying numbers of MoDCs, monocytes, and CD1b/c⁺, CD16⁺, BDCA-3⁺, CD123⁺, and CD34⁺ Lin⁻ cells were cultured for 5 days in a 96-well plate with 10⁵ allogeneic normal peripheral blood T cells per round-bottom well. T-cell proliferation was assessed by addition of tritiated thymidine. Results are expressed as means ± SEM of triplicate wells. Three separate representative experiments (A-C) are shown. Data are representative of a minimum of 3 experiments for each subset compared to the CD1b/c⁺population.