Differential survival of Lin⁻HLA-DR⁺ PBMC subpopulations.

(A) The frequency of CD34⁺ or CD16⁺ cells was examined in freshly sorted or cultured (18 hours with GM-CSF and IL-3) Lin⁻ CD11c⁻ or CD11c⁺ cells, respectively. The preferential survival of CD34⁺ cells over CD123^hi (top panel) and reduced frequency of the CD11c⁺CD16⁺ subset (bottom panel) following culture were noted. (B) The frequency of dead cells was examined in 18-hour cultures of sort-purified CD11c⁺CD16⁻and CD11c⁺ (CD16⁺ inclusive) populations. An increase in the percentage of PI⁺ (dead) cells was associated with the CD11c⁺ (CD16⁺inclusive) cultures.