Phenotypic analysis of Lin⁻ PBMC preparations identifies 5 phenotypic subsets.

CD56-depleted Lin⁻ preparations were stained with FITC-SAM and either CD16, CD1b/c, BDCA-3, CD123, or CD34. Live cells were gated based on forward- and side-scatter characteristics and analyzed for (A) CD16, (B) CD1b/c, (C) BDCA-3, (D) CD123, and (E) CD34 staining. Sort-purified Lin⁻HLA-DR⁺ cells were used to examine the relative intensity of HLA-DR and CD11c on each of the defined subsets: CD16 (F,K), CD1b/c (G,L), BDCA-3 (H,M), CD123 (I,N), and CD34 (J,O). Differences in fluorescence intensity for BDCA-3 (H,M) or CD11c (K-O) reflect the use of different fluorescent conjugates.