Fig. 6.
![Fig. 6. In vitro phosphorylation of cortactin by PAK. / PAK was immunoprecipitated (IP) from platelets stimulated or not with THR (0.5 UI/mL) as indicated and incubated with 5 μg purified rHu cortactin in the presence of 20 μM MgCl2-ATP and 0.185MBq (5 μCi) γ[32P]-ATP. Phosphocortactin (pCortactin) was revealed by autoradiography. PAK was quantified by anti-PAK1/2 immunoblot (IB). Results are representative of 3 separate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/13/10.1182_blood.v100.13.4462/4/h82423531006.jpeg?Expires=1722716612&Signature=X04ZYmgCu0H1vEXpUbuWjsbGlwBh58A8XlLJW5sm1Aiz2l9Em~k8o0MylMDEfgzoiRXMTmpv6~RnBezusFeEqDV2AYgV-52SpsQ4EXAixIjzgj-2QarrDIWgw3R6bOGRb7Kun4Ua~nioQKvoILhDANz1AHmVRulXEzbU3RCI3a7mpIwg4ROnyhcAoNK~tsmx9c~t1BRixVWjS-EHqz73hJHrRCTuVggPBkk~yUvEww9WNfC3gAxbddYFn2Jnf~-dB2Wo4msu01wZuNYFZyDdVz9r5r6R~zlid0LcN6RMTp~btPbwiMjfosx6MocxANf5Ppex2xqV8v-idoDF22pItw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
In vitro phosphorylation of cortactin by PAK.
PAK was immunoprecipitated (IP) from platelets stimulated or not with THR (0.5 UI/mL) as indicated and incubated with 5 μg purified rHu cortactin in the presence of 20 μM MgCl2-ATP and 0.185MBq (5 μCi) γ[32P]-ATP. Phosphocortactin (pCortactin) was revealed by autoradiography. PAK was quantified by anti-PAK1/2 immunoblot (IB). Results are representative of 3 separate experiments.
