Detection of FLT3 ITD in younger adults with AML.

Exons 14 and 15 of the FLT3 gene were PCR amplified from genomic DNA, and PCR products were resolved on a 2% agarose gel stained with ethidium bromide. Arrow indicates the normalFLT3 gene product (328 bp). High-molecular–weight bands represent FLT3 ITDs. Sample 1 shows a strong mutant band and loss of the wild-type allele. M indicates 100-base pair DNA ladder (Gibco BRL); 1-12, samples from 12 different AML patients.