Human CD34 genomic fragments extending from 18 to 48 kb of upstream sequence coexpress with early hematopoietic surface markers murine CD34, c-Kit, and Sca-1.

(A) Fluorescence cytometry was performed as described in Figure 3, comparing 4 different founders representing 4 different genomic fragments described in Figure 1. The “18 kb 5′ upstream sequence construct” represents MluI-digested PAC54A19, which includes 18 kb of upstream and 43 kb of downstream flanking sequences (Figure 1). (B) Fluorescence cytometry was performed as described using bone marrow from the 70-kb PvuI-digested PAC54A19 construct, which includes 18 kb of upstream and 25.7 of downstream flanking sequences (Figure 1).