Fig. 4.
FcεRI-induced tyrosine phosphorylation of cellular proteins is not affected by the overexpression of 3BP2-SH2 domain.
Control cells (pMT3) and cells overexpressing SH2 domain of 3BP2 were primed with anti-DNP IgE and stimulated with 10 ng/mL antigen DNP-BSA for the indicated times. Total cell lysates (105 cell equivalents per lane) were separated by 10% SDS-PAGE and analyzed by immunoblotting with anti-pTyr mAb. Similar results were obtained when the other lines were examined.
