Fig. 2.
Fig. 2. Ex vivo expansion ability of Fanca-deficient bone marrow cells. / (A) Total bone marrow cells from Fanca+/+(■) and Fanca−/− (▪) cells were subjected to ex vivo expansion in the presence of 3 different combinations of growth factors. At weekly intervals, cells were counted and diluted to the input concentration (105 cells/mL). Data represent the total cell number generated by the initial 105 cells. Each point represents the mean ± SEM corresponding to 4 independent experiments. *Statistical significance betweenFanca+/+ and Fanca−/−samples at P < .05. (B) Lin−Sca-1+ cells fromFanca+/+ (○) and Fanca−/−(●) were purified and ex vivo expanded with SCF and IL-11. Cytometry histograms of bone marrow cells before and after purification of the Lin−Sca-1+ population and the ex vivo expansion kinetics following SCF and IL-11 stimulation are shown. The number in the histogram indicates the percentage of cells within the statistics quadrant.

Ex vivo expansion ability of Fanca-deficient bone marrow cells.

(A) Total bone marrow cells from Fanca+/+(■) and Fanca−/−(▪) cells were subjected to ex vivo expansion in the presence of 3 different combinations of growth factors. At weekly intervals, cells were counted and diluted to the input concentration (105 cells/mL). Data represent the total cell number generated by the initial 105 cells. Each point represents the mean ± SEM corresponding to 4 independent experiments. *Statistical significance betweenFanca+/+ and Fanca−/−samples at P < .05. (B) Lin−Sca-1+ cells fromFanca+/+ (○) and Fanca−/−(●) were purified and ex vivo expanded with SCF and IL-11. Cytometry histograms of bone marrow cells before and after purification of the Lin−Sca-1+ population and the ex vivo expansion kinetics following SCF and IL-11 stimulation are shown. The number in the histogram indicates the percentage of cells within the statistics quadrant.

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