B-cell subsets in normal mice.

BM, spleen, and PC lavage cells from C57BL/6 mice were harvested and stained with anti-B220 and anti-CD23 mAbs (BM); anti-B220, anti-CD21/35, and anti-HSA mAbs (spleen); or anti-IgM and anti-CD23 mAbs (PC) and analyzed by flow cytometry. The spleen CD21/35 vs HSA contour plot (panel B) is derived from B220⁺gated cells.