Fig. 1.
Frequency analysis of single-pass sequences.
Clones from the primary myeloma libraries were plated, randomly picked, and PCR amplified. Sequencing of the 5′ end of cDNAs was performed on 2 μL of PCR products using primers nested within the forward PCR primer. Sequence data generated were compared using the Blast algorithm9 against Ref Seq, nonredundant GenBank/EMBL/DDBJ, high-throughput genomic sequence (htgs), dbEST, and Unigene databases on Pentium Pro200 Solaris x 86 platform (Micron Electronics). Assignment of putative identities required a minimum Blastn E value = 10−10. Each clone was classified based on Blast result from database searches (A) or according to functional categories (B).
