JAM-2 expression on human PBLs.

Cytofluorometric analysis of JAM-2 was performed on human PBLs. Antibodies used for staining were biotinylated anti–JAM-2 in combination with either (A) anti-CD3 directly conjugated to FITC, (B) anti-CD19 directly conjugated to FITC, or (C) anti-CD14 directly conjugated to FITC, as indicated on the dot-plot axes. The appropriate forward and side scatter gates were used to display lymphocytes (A,B) or monocytes (C).