Hypoxia-inducible EPO gene expression in human NB cell lines.

SH-SY5Y cells, Kelly cells, and the hepatoma cell line HepG2 were grown for 24 hours under normoxic (NOX), hypoxic (HOX), or anoxic (AOX) conditions. Total RNA was prepared, reverse-transcribed into cDNA, and subjected to real-time PCR for EPO and β-actin quantification. EPO cDNA was normalized to β-actin cDNA and expressed as relative units × 1000. Bars represent mean cDNA values (± SD) from 3 separate experiments in which each cDNA was quantitated in triplicate.