Fig. 1.
Fig. 1. Measurement of mRNA signals for GATA-3 by RT-PCR. / Pre-G or post-G CD4+ T cells from the same donor were activated with allogeneic TCD PBMCs for 7 days in MLR at a 1:3 stimulator-responder ratio. (A) A representative experiment is shown. TH1 and TH2 clones were established after phytohemagglutinin activation of normal peripheral blood T cells in the presence of IL-12 (5 ng/mL) plus anti–IL-4 antibodies (200 ng/mL; TH1-promoting condition) or IL-4 (200 IU/mL) plus anti–IL-12 antibodies (10 μg/mL; TH2-promoting condition). (B) Error bars represent median values and ranges recorded in 6 independent experiments; band intensity was expressed as absorbance units relative to the housekeeping gene aldolase. *P < .05 compared with pre-GMLRCD4+ T cells.

Measurement of mRNA signals for GATA-3 by RT-PCR.

Pre-G or post-G CD4+ T cells from the same donor were activated with allogeneic TCD PBMCs for 7 days in MLR at a 1:3 stimulator-responder ratio. (A) A representative experiment is shown. TH1 and TH2 clones were established after phytohemagglutinin activation of normal peripheral blood T cells in the presence of IL-12 (5 ng/mL) plus anti–IL-4 antibodies (200 ng/mL; TH1-promoting condition) or IL-4 (200 IU/mL) plus anti–IL-12 antibodies (10 μg/mL; TH2-promoting condition). (B) Error bars represent median values and ranges recorded in 6 independent experiments; band intensity was expressed as absorbance units relative to the housekeeping gene aldolase. *P < .05 compared with pre-GMLRCD4+ T cells.

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