American Society of Hematology

Fig. 2.

$Fig. 2. In vivo SCL induction of c-kit gene expression in developing B cells. / Bone marrow–derived B220+ cells (A) or fractionated B-cell precursors (B) from SIL-SCL transgenic mice (SCLtg/tg) exhibit increased c-kit expression. B lineage cells from WT and SCLtg/tg were purified by flow cytometry,31 and gene expression was assessed by RT-PCR. RAG-2 expression was monitored to confirm the identity of purified B cells, and S16 was used as a control for the amount of cDNA. Thec-Kit mRNA levels were quantified by means of ImageQuant software (Molecular Dynamics, Sunnyvale, CA) and normalized with the use of S16 signals.$

In vivo SCL induction of c-kit gene expression in developing B cells.

Bone marrow–derived B220⁺ cells (A) or fractionated B-cell precursors (B) from SIL-SCL transgenic mice (SCL^tg/tg) exhibit increased c-kit expression. B lineage cells from WT and SCL^tg/tg were purified by flow cytometry,31 and gene expression was assessed by RT-PCR. RAG-2 expression was monitored to confirm the identity of purified B cells, and S16 was used as a control for the amount of cDNA. Thec-Kit mRNA levels were quantified by means of ImageQuant software (Molecular Dynamics, Sunnyvale, CA) and normalized with the use of S16 signals.

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