Restriction map of the human CD34 locus and PAC clones.

(A) The top diagram depicts the human CD34 genomic locus. Numbering is relative to the transcription start site (TSS).22 Black lines represent the 8 exons. Shown above are the restriction sites mapped in panel B. Arrowheads indicate the location of the 5′ and 3′ primer sets used to screen the PAC library (the 3′ primer set was used for genotyping). Shown below are diagrams of the inserts of the 3 human CD34 PAC clones; the horizontal arrows refer to the transcription start site. Vertical arrow represents the location of theSfiI restriction site used to linearize PAC-88L2 prior to generation of transgenic mice. Also shown is the relative location of exons 11-14 of the CD46 gene, which is included in the 3′ end of PAC-7H11 in opposite transcriptional orientation to theCD34 gene. (B) A representative example of field inversion gel electrophoresis (FIGE) which was employed to map 1 μg DNA from each PAC clone. Migration of Lambda HindIII and MidRange I markers (NEB, Beverly, MA) are shown on the left. The lane marked “8-48 kb” shows markers ranging from 8 kb to 48 kb (BioRad, Hercules, CA).