Fig. 2.
Fig. 2. Synthesis rates of primary translated peptide of FV. / COS-1 cells coexpressing wt-FV or the FV mutants together with wild-type protein S as a control were radiolabeled for 30 minutes. Radiolabeled FV and protein S in cell lysates were quantitatively immunoprecipitated and electrophoresed on polyacrylamide gels. To correct for transfection and radiolabeling efficiency among wells, the FV–to–protein S ratio was calculated by measuring radioactivity of FV- and protein S–specific bands on the gels. The histograms and bars represent the mean ± SD (n = 3). The mean value of wt-FV is assigned to be 100% in this figure. 385 indicates Met385Thr; 1299, His1299Arg; 1736, Met1736Val; 2194, Asp2194Gly.

Synthesis rates of primary translated peptide of FV.

COS-1 cells coexpressing wt-FV or the FV mutants together with wild-type protein S as a control were radiolabeled for 30 minutes. Radiolabeled FV and protein S in cell lysates were quantitatively immunoprecipitated and electrophoresed on polyacrylamide gels. To correct for transfection and radiolabeling efficiency among wells, the FV–to–protein S ratio was calculated by measuring radioactivity of FV- and protein S–specific bands on the gels. The histograms and bars represent the mean ± SD (n = 3). The mean value of wt-FV is assigned to be 100% in this figure. 385 indicates Met385Thr; 1299, His1299Arg; 1736, Met1736Val; 2194, Asp2194Gly.

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