ABCG2 and β-actin mRNA expression in cell lines by Northern analysis.

Total RNA was isolated from parental AML3 cells, clone 3.3, and clone 6.2. RNA was separated on a formaldehyde/agarose gel and transferred to a nylon membrane, then probed for ABCG2 mRNA (top row). The membrane was then stripped and probed for β-actin (bottom row).