Nramp2/DMT1 localizes around phagocytosed spermatozoids in TM4 cells.

Biotinylated spermatogenic cells were fed to TM4 cells before fixation and staining with FITC-avidin and anti-Nramp2/DMT1 antiserum and a rhodamine conjugated secondary antibody. Slides were analyzed by confocal microscopy, and images were overlaid. Phase-contrast images of the cells and a z-axis centered on the phagocytosed sperm are included.