Nramp2/DMT1 is associated with erythrocyte-containing phagosomes in RAW macrophages.

(A) Crude membrane extracts (m) from control c-Myc–Nramp1 CHO, c-Myc–Nramp2/DMT1, c-Myc–Nramp1 RAW cells, RBCs containing phagosomes, or latex bead–containing phagosomes (p), prepared from, respectively, c-Myc–Nramp2 RAW and RAW cells, were analyzed for anti–c-Myc reactivity. (B) Crude membrane extracts (m) from c-Myc–Nramp1 CHO or RAW cells or RBCs containing phagosomes from c-Myc–Nramp2 RAW cells (p) were sequentially probed with goat anti-EEA1 and rabbit anti–Rab 7 antibodies. (C) Crude membrane extracts (m) from RAW or untransfected CHO cells or RBCs containing phagosomes from c-Myc–Nramp2 RAW cells and latex bead–containing phagosomes from RAW cells (p) were analyzed with rabbit anti–cathepsin D and anti–Rab 7 antibodies.