Subcellular localization of Nramp2/DMT1 and Lamp1 in Sertoli cells after phagocytosis of latex particles.

TM4 cells were fixed and stained with anti-Lamp1 followed by an FITC-conjugated secondary antibody (green) and anti-Nramp2/DMT1 with rhodamine conjugated secondary antibody (red), either before (upper panel) or 1 hour after phagocytosis of latex beads (lower panel). Cells were examined under a ×63 objective by confocal microscopy. Rhodamine (red, Nramp2/DMT1) and FITC (green, Lamp1) staining were overlaid (yellow shows overlap).