Fibrinogen-mediated platelet aggregation depends on PDI.

Effect of the monoclonal anti-PDI clone RL90 on agonist-induced platelet aggregation. PRP was incubated with anti-FcγRII antibody clone IV.3. Monoclonal anti-PDI, clone RL90, or isotype-matched control mouse IgG in ascites fluid diluted 1:500 in saline was added to the PRP. Aggregation induced by the agonists indicated was assessed after 10 minutes of incubation. Shown is one representative experiment of 3.