Morphologic analysis of NK/DC clusters from NB patients.

Cytospins were carried out with cells harvested in the different cocultures described in the Figure 5 legend (1:1 ratio, day 7), stained with May-Grünwald-Giemsa coloration and observed under optical microscopy: NK cells/iDCs (panel A); NK cells/iDCs with LPS (panels B and E); NK cells/mDCs (panel C); NK cells alone without IL-2 (panel D); and NK cells alone with IL-2 (1000 IU/mL) (panel F). Original magnification was × 1000 for all panels, except that it was × 400 for panel E. These observations are representative of 2 experiments.