Fragments of α-synuclein have no effect on ionomycin-induced PF4 release from platelets.

(A) Diagram of the wild-type α-synuclein and its deletion mutants. (B) Purified proteins of α-synuclein, α-synuclein fragments (Syn1-97, Syn61-140, Syn96-140) were separated on 12% SDS polyacrylamide gel and stained with Coomassie brilliant blue R-250. (C) Both N-terminally and C-terminally truncated α-synuclein proteins did not appear to inhibit the ionomycin-induced PF4 release from platelets. For these experiments 10 μM synuclein proteins was used, and the PF4 released was measured as in Figure 1.