Platelet adhesion to decorin stimulates protein tyrosine phosphorylation.

Samples of whole cell lysates from adherent (A) and nonadherent (NA) platelets to decorin or BSA-coated dishes containing the same amount of proteins (10 μg) together with a sample of resting untreated platelets (TOT) were separated by SDS-PAGE on a 7.5% acrylamide gel. Proteins were transferred to nitrocellulose and probed with antiphosphotyrosine antibody. The positions of standard molecular weight markers are reported on the left.