Effect of platelet stimulation on adhesion to decorin.

Samples of gel-filtered platelets were mixed with buffer (none), 1 mM peptide GRGDS, 10 μM ADP, or 10 μM thrombin receptor activating peptide (TRAP) in the presence of 1 mM GRGDS and immediately added to decorin-coated plates. After incubation for 90 minutes, the percentage of adherent platelets was determined by a colorimetric assay. Results are the mean ± SD of 3 to 4 separate experiments.