![Fig. 6. The amount of μ-calpain is significantly higher in the B-CLL cells than in healthy lymphocytes. / The expression of μ-calpain was assessed by Western blotting in the samples of B-CLL cells and of PBLs and enriched CD3− cells of healthy controls. (A) A representative blot of 1 of 3 experiments performed (containing data from 3 healthy people [CD3− 1 through CD3− 3] and 3 different, age-matched B-CLL patients [B-CLL1 through B-CLL3]). Upper row: expression of μ-calpain; lower row: expression of F-actin in the same material as described in “Patients, materials, and methods.” (B) Graphical summary of all 3 experiments. Bars represent arithmetical means of the amount of protein in the μ-calpain bands, expressed as arbitrary densitometric units per milligram of cellular protein ± SEM. Asterisks indicate statistical significance of difference (Student t test) between the adjacent bars (B-CLL, CD3−) at the level of P < .01 (*).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/5/10.1182_blood-2001-11-0073/3/h81723046006.jpeg?Expires=1722785262&Signature=yA~17QpNkCNbGGEc77BjLhKkTeYU4xJM5aiY2fbn6~i-tZc7LPSVJ4wjXK4h3sVEgl3sGIOR0T0N~Il2QzPr9LnUqIYGmJsAEJ3SP91nBzTj-sFwYWjbY~IVvTf6W4q0pjBYwlsRfLEFMU-EffsQHhfyLkeGSkHxh9ppshJc-yMXpZ97o0dOjwVEAs7bGbR3rL5izEXidpFeek1JePMfJPJFWi2ZEMdZq~2F83T5KPvg-h-IMB~4~CgW3TzcSAaNuTBFgXpskYQGfhPpLovaKqU542XoIVIxZyrisb4crlQN7uJo25xqk9cPqryWtQG3sw5s10cots6sizvyDgpzVg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The amount of μ-calpain is significantly higher in the B-CLL cells than in healthy lymphocytes.
The expression of μ-calpain was assessed by Western blotting in the samples of B-CLL cells and of PBLs and enriched CD3− cells of healthy controls. (A) A representative blot of 1 of 3 experiments performed (containing data from 3 healthy people [CD3− 1 through CD3− 3] and 3 different, age-matched B-CLL patients [B-CLL1 through B-CLL3]). Upper row: expression of μ-calpain; lower row: expression of F-actin in the same material as described in “Patients, materials, and methods.” (B) Graphical summary of all 3 experiments. Bars represent arithmetical means of the amount of protein in the μ-calpain bands, expressed as arbitrary densitometric units per milligram of cellular protein ± SEM. Asterisks indicate statistical significance of difference (Student t test) between the adjacent bars (B-CLL, CD3−) at the level of P < .01 (*).
