Expression of cytokine and chemokine mRNA in skin lesions.

RNA was harvested from skin biopsies of a patient with autologous GVHD and 4 patients with allogeneic GVHD. cDNA were analyzed for IL-10, IFN-γ, IL-2, IL-4, MIP-1α, and RANTES by real-time PCR. Data were normalized against GAPDH. Levels of cytokine and chemokine mRNA expression in PBMCs from healthy individuals were shown as the baseline control.