Expression of chemokine mRNA in PBMCs.

RNA was harvested from PBMCs of healthy individuals (black bars, n = 8), patients evaluated at the onset of autologous GVHD (white bars, n = 17 different time points for 15 patients), and patients who did not develop autologous GVHD (gray bars, n = 43 points for 21 patients) (A). cDNA was analyzed for chemokine transcripts including MIP-1α, regulated upon activation in normal T cells, expressed and secreted (RANTES), interferon-gamma, and IP-10 by real-time PCR. Temporal analysis of MIP-1α and IP-10 mRNA levels for patients who developed autologous GVHD (white bars) and patients who did not (gray bars) is presented in B and C. Chemokine mRNA levels were normalized against GAPDH.