Fig. 3.
Temporal relationship between cytolytic activity and cytokine expression.
The changes in the cytotolytic activity and cytokine mRNA expression over time were assessed in PBMCs from a patient who developed autologous GVHD following transplantation. Cytotoxicity against autologous PHA-blasts and the T47D breast cancer cell line was measured using a standard 51Cr release assay at a 100:1 effector-to-target ratio. Relative expression of IL-10 and IFN-γ mRNA levels normalized against GAPDH were determined by real-time PCR.
