Temporal analysis of cytokine transcription in PBMCs.

PBMCs from the patients on the autologous GVHD induction protocol were serially monitored for IL-10 (A), IFN-γ (B), IL-2 (C), and IL-4 (D) gene expression by real-time PCR during the course of treatment (day 12 through day 33). Gene expression was determined in PBMCs of 36 patients at 4 different time points (days 12, 19, 25, and 33 after transplantation) and compared with patients evaluated at onset of autologous GVHD (white bars) and patients who did not develop autologous GVHD (gray bars). *P < .05; patients evaluated at the onset of autologous GVHD versus patients who did not develop autologous GVHD.