Fig. 6.
Transactivation of bystander CLL cells after coincubation with CD40L-transduced CLL cells.
CLL cells (1 × 105) transduced with AAV/CD40L were coincubated with equal amounts of naive CLL cells labeled with a fluorescence dye (CellTracker). CD80 expression on naive bystander leukemic cells was detected 72 hours later by flow cytometry (black bars). In control assays anti–murine CD40L mAb was added to the mixture of transduced and bystander CLL cells before CD80 expression was detected 72 hours later (gray bars). As further controls uninfected (striped bars) or wild-type AAV-infected cells (open bars) were used for stimulation of bystander CLL cells.
