Genomic titers of rAAV.

AAV/EGFP preparations and the vector plasmid pEGFP were blotted in a serial 2-fold dilution and hybridized with a random-primed transgene specific probe by standard methods. Particle titers were determined by comparing the intensity of the hybridization signals with that obtained for the vector plasmid standard of known concentration blotted on the same membrane. A DNA fragment encoding resistance for neomycin served as negative control in the hybridization reaction. DNAse treatment (0.5 μg/μL DNAse I, Boehringer Mannheim; 60 minutes at 25°C) was performed to remove free, unpackaged viral genomes.