![Fig. 5. Effect of Mcl-1 antisense oligonucleotides on HK protection against spontaneous apoptosis. / B-CLL cells from a representative patient specimen were cultured for 24 hours alone or together with HK, in the presence or absence of 1 to 5 μM antisense (AS) or random (scrambled [SC]) control oligonucleotides. Oligonucleotides were introduced into CLL B cells by electroporation (750 to 1250 V/cm and 900 μF) prior to coculture. The CLL B cells were recovered from cultures, and the percentage of apoptosis was determined by annexin V–FITC/PI double staining, with the use of flow cytometric analysis (n = 4). Simultaneously, protein-containing lysates were prepared, and 12.5 μg per lane protein from each lysate was analyzed by SDS-PAGE/immunoblot assay with the use of antibodies specific for Mcl-1 or β-actin. (One representative experiment of a total of 4 is presented.)](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/5/10.1182_blood.v100.5.1795.h81702001795_1795_1801/3/h81723070005.jpeg?Expires=1725054380&Signature=tEC5FYyTVjyJ8zDkeEscSiq-ElpqNx4ZbdMth2hIpschQYfl7UiGk0hK26uwjqdfHfRtaWJ85agb1NFdk-v3ZX-~0tHmPJ4Np7hBH-OX13IL8jFACrAzG2DrAshXvhNehWPNKUhTg~zGgVdaVduIiXuW8OAIH4pg2JugXyY5eGjBWv5IadfbqkUNTQKzYiQG7msOW6ZIA7ZL9fDhMmLUdn0i32l7CtL11yBBcGzcTu5eOwj~Y-g~o30wU2rKtSDjB7UlbjJZey~9kRhYJKY0lYX03y3bMMYq0zr7--weoG0V6zZVLnXYdX2iGWUc~ylz3JmbQv0NauXTDYtiABZdNQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of Mcl-1 antisense oligonucleotides on HK protection against spontaneous apoptosis.
B-CLL cells from a representative patient specimen were cultured for 24 hours alone or together with HK, in the presence or absence of 1 to 5 μM antisense (AS) or random (scrambled [SC]) control oligonucleotides. Oligonucleotides were introduced into CLL B cells by electroporation (750 to 1250 V/cm and 900 μF) prior to coculture. The CLL B cells were recovered from cultures, and the percentage of apoptosis was determined by annexin V–FITC/PI double staining, with the use of flow cytometric analysis (n = 4). Simultaneously, protein-containing lysates were prepared, and 12.5 μg per lane protein from each lysate was analyzed by SDS-PAGE/immunoblot assay with the use of antibodies specific for Mcl-1 or β-actin. (One representative experiment of a total of 4 is presented.)
