Effect of dendritic cells on CLL B-cell apoptosis and Mcl-1 expression.

(A) CLL B cells were cultured alone or together with either HK or normal dendritic cells. Apoptosis was measured by annexin V–FITC/PI double staining by means of flow cytometric analysis at various times after initiation of culture (n = 5). (B) Protein lysates were prepared after 24 hours and analyzed by SDS-PAGE/immunoblot assay with the use of antibodies specific for Mcl-1 and β-actin.