Fig. 3.
Effect of HK cells on levels of Mcl-1 in CLL B cells.
(A) CLL B cells from representative patient specimens were cultured for 24 hours alone (“control”); with HK cells; with NIH3T3 control cells; or with NIH3T3-hCD40L cells. CLL B cells were recovered from cultures, and protein-containing lysates were prepared, normalized for total protein content (12.5 μg per lane) and analyzed by SDS-PAGE/immunoblot assay with the use of antibodies specific for Mcl-1, Bcl-XL or β-actin. (B) Mcl-1 and β-actin protein levels were assayed by SDS-PAGE and immunoblotted as above for 6 CLL B-cell samples. Data were quantified by scanning densitometry and expressed as a ratio (n = 6).
