FANCE is required for the nuclear accumulation of FANCC.

(A) Whole-cell extracts were prepared from the indicated lymphoblast cell lines. Cellular proteins were immunoblotted with anti-FANCD2, anti-FANCC, or anti-HA antibody. (B) Subcellular localization of FANCC in lymphoblast cell lines. Equal amounts of cytoplasmic (C) and nuclear (N) proteins from the indicated lymphoblast lines were immunoblotted with anti-FANCC, anti–β-tubulin, or anti–topoisomerase II antibody, as indicated.