Effect of the expression of Cdc42N17 on MAP kinase activation.

(A) Differentiated induced or uninduced cells were stimulated for various periods of time with fMLFK or PMA. Erk1/2 phosphorylation was detected after SDS-PAGE by Western immunoblotting with a phosphospecific antibody. (B) A fraction of an unstimulated cell lysate was probed with an anti-Erk2 antibody. Results are representative of 3 independent experiments.