![Fig. 6. Effect of the expression of Cdc42N17 on the activation of PLCβ2. / (A) fMLFK-mediated IP3 formation. IP3 levels in differentiated Cdc42N17-expressing cells (black bars) and uninduced cells (gray bars) were determined at time intervals after stimulation with fMLFK (1 μM) using an inositol-1,4,5-trisphosphate [3H] radioreceptor assay kit. Error bars represent the standard deviation of 3 independent experiments performed in duplicate. (B, C) Kinetics of fMLFK-mediated intracellular calcium mobilization. Cells were loaded with Fura-2, and fMLFK-mediated calcium increase was assayed in the absence of extracellular calcium. Mean responses ± SE (n = 7) in differentiated Cdc42N17- (B) or Cdc42V12- (C) expressing cells grown in the absence (triangles) or in the presence (circles) of doxycycline are presented. (D) Effect of ionomycine on superoxide production in Cdc42N17-expressing HL-60 cells. Cells grown and differentiated in the absence of doxycycline were treated or not for 2 minutes with 1 μM ionomycine at 37°C before stimulation with fMLFK. Data are representative of 2 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/5/10.1182_blood-2001-12-0193/3/h81723025006.jpeg?Expires=1724244059&Signature=hbCpsdJ7QSVeL2g31LZa9lcOeGnKR7NBcwUf41ckQxRV2pWwN1y7AcFmObkNHom5~y1JJWCRlQKncwq5FSQDj3icBqTdbDuqiuefkMiZzPOe6BOXtXqfzFWP2ZIJp4kcKPC0gsIo7MKou6QdAdjbJJ34xd-415sPk3slEB0uOf1QCopAUEauIMQqAxxAc17KrbjEQiVzn4u9AK8JUkmix41bJCwW5z1~YsqavRooMuDN0T15zEbWidyJFYg7J6Wuo99O~o~RdZLXgxhZ4mtIJaFg8CKtsiXs5CsIhQCtTLCfXas15RWA1VS6DuoSHMXAgnEq-Xsug-2f0xc8EWIjfw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of the expression of Cdc42N17 on the activation of PLCβ2.
(A) fMLFK-mediated IP3 formation. IP3 levels in differentiated Cdc42N17-expressing cells (black bars) and uninduced cells (gray bars) were determined at time intervals after stimulation with fMLFK (1 μM) using an inositol-1,4,5-trisphosphate [3H] radioreceptor assay kit. Error bars represent the standard deviation of 3 independent experiments performed in duplicate. (B, C) Kinetics of fMLFK-mediated intracellular calcium mobilization. Cells were loaded with Fura-2, and fMLFK-mediated calcium increase was assayed in the absence of extracellular calcium. Mean responses ± SE (n = 7) in differentiated Cdc42N17- (B) or Cdc42V12- (C) expressing cells grown in the absence (triangles) or in the presence (circles) of doxycycline are presented. (D) Effect of ionomycine on superoxide production in Cdc42N17-expressing HL-60 cells. Cells grown and differentiated in the absence of doxycycline were treated or not for 2 minutes with 1 μM ionomycine at 37°C before stimulation with fMLFK. Data are representative of 2 independent experiments.
