Cdc42N17 has no effect on PI3Kγ activation.

(A) HL-60 cells expressing Cdc42N17 were differentiated with Bt₂cAMP in the presence or absence of doxycycline and stimulated for various periods of time with fMLFK. The PI3Kγ-downstream effector Akt was selectively immunoprecipitated from cell lysates. In a kinase assay, phosphorylation of the Akt substrate GSK-3 was measured by Western immunoblotting using a phospho-GSK3 antibody. Results are representative of 2 independent experiments. (B) Western blot analysis with anti-Akt antibody was performed to assess the amount of immunoprecipitated Akt kinase.