Analysis of wild-type RD cells (left panels) and RD cells transfected with the

PAX3-FKHR fusion gene (right panels). (A) Expression of CXCR4 by FACS. (B) Expression of CXCR4 mRNA by RNase protection. (C) Phosphorylation of MAPK p42/44 by SDF-1β. (D) Directional chemotaxis to SDF-1β. Original magnification, × 400. (E) Calcium flux in response to SDF-1β or FBS. The experiments were repeated 3 times with similar results.